Coding

Part:BBa_M36914:Design

Designed by: Rohit Talreja, Kelly Nguyen, Jacqueline Tao   Group: Stanford BIOE44 - S11   (2012-12-06)


Codes for the benzylalcohol acetyltransferase enzyme


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 58
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 58
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 58
    Illegal NgoMIV site found at 1203
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was extracted from a flower, it was optimized for expression in E. coli


Source

The cDNA and amino acid sequences for this part come from a research paper by Dudareva et. al Dudareva, Natalia, John C. D'Auria, Kyoung Hee Nam, Robert A. Raguso, and Eran Pichersky. "Acetyl-CoA:benzylalcohol Acetyltransferase - an Enzyme Involved in Floral Scent Production in Clarkia Breweri." The Plant Journal 14.3 (1998): 297-304.

References