Coding
Part:BBa_M36914:Design
Designed by: Rohit Talreja, Kelly Nguyen, Jacqueline Tao Group: Stanford BIOE44 - S11 (2012-12-06)
Codes for the benzylalcohol acetyltransferase enzyme
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 58
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 58
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 58
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 58
Illegal NgoMIV site found at 1203 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part was extracted from a flower, it was optimized for expression in E. coli
Source
The cDNA and amino acid sequences for this part come from a research paper by Dudareva et. al Dudareva, Natalia, John C. D'Auria, Kyoung Hee Nam, Robert A. Raguso, and Eran Pichersky. "Acetyl-CoA:benzylalcohol Acetyltransferase - an Enzyme Involved in Floral Scent Production in Clarkia Breweri." The Plant Journal 14.3 (1998): 297-304.